Ukucocwa ngokufanelekileyo kweeNgcebiso zePipette kunye neePipettes, uFakelo kunye nokusetyenziswa kwePipette

3. Imibhobho yoxinaniso oluphezulu/iisampulu zoxinaniso oluphantsi
Amaxabiso achanekileyo epipette asekelwe ekudluliseleni amanzi acocekileyo.Ukuba ubuninzi besampulu buhluke kakhulu kubuninzi bamanzi, ngoko ukuchaneka kuya kuba kubi kakhulu ngokuhambelanayo.Ngoko ke, kuyimfuneko ukufumanisa ubuninzi besampulu ngaphambi kokubhobhoza, kwaye emva koko ulungelelanise uluhlu kwimveliso yomthamo wesampuli ukuba idluliselwe kunye nobuninzi.
Ngokomzekelo, ukuba ubuninzi besampuli ngu-1.2 g / cm3 kwaye kufuneka udlulise i-300 ul, kufuneka ubeke uluhlu kwi-360 ul.Le yindlela yokulungelelanisa kuphela, kodwa indlela yokulungelelanisa engqongqo idinga ukusetyenziswa kwe-gauge okanye ibhalansi njengesixhobo esincedisayo sokubala ngokuchanekileyo.

4. Ukususa iisampulu eziphezulu nezisezantsi zobushushu
Amanqaku amathathu kufuneka aqatshelwe xa kufakwe iisampulu zobushushu obuphezulu / obuphantsi.
Okokuqala, ungalokothi umanzise iingcebiso ze-pipette ngaphambi kokubhobhoza.
Okwesibini, i-pipetti entsha kufuneka isetyenziswe kwi-pipette nganye.
Okwesithathu, funa kwaye ukhuphe ngokukhawuleza.

Iingcebiso zokusetyenziswa kwepipette

1. Sebenzisa i-pipetti efanelekileyo: Ukuqinisekisa ukuchaneka okungcono kunye nokuchaneka, kucetyiswa ukuba umthamo wepayipi ungaphakathi kwe-35% ukuya kwi-100% yoluhlu lwe-pipette tip.

2. Ufakelo lwengcebiso yePipette: Ngobuninzi beempawu zeepipettes, ngokukodwa i-pipettes ye-multichannel, ukufaka iingcebiso akuwona umsebenzi olula: ukufezekisa isitywina esilungileyo, isibambo se-sleeve se-pipette kufuneka sifakwe kwi-tip kwaye sigxininiswe ngokuyijika ngakwesobunxele kwaye ekunene okanye ukuyishukumisa emva naphambili.Abanye abantu baphinde basebenzise i-pipette ukuqinisa iingcebiso ngokuzibetha ngokuphindaphindiweyo, kodwa oku kunokukhokelela ekuphazamisekeni kweengcebiso kunye nokuchaphazela ukuchaneka, okanye kwiimeko ezinzima zonakalise i-pipette, ngoko kufuneka igwenywe.

3. I-angle yokuntywiliselwa kwingcebiso kunye nobunzulu: I-engile yokuntywiliselwa kwincam kufuneka igcinwe ngaphakathi kwe-20 degree tilt kwaye kufuneka igcinwe ime nkqo;Incam yokuntywiliselwa ubunzulu bukhuthazwa ngolu hlobo lulandelayo: Ubungakanani bePipette ubunzulu beNcam yokuntywiliselwa 2 µL kunye ne-10 µL 1 mm 20 uL kunye ne-100 uL 2-3 mm 200 uL kunye ne-1000 uL 3-6 mm 5000 µL kunye ne-10 mL 6-10 mm

4. Ingcebiso yokuhlanjululwa: Kwiisampuli eziqhelekileyo zokushisa, i-tip yokuhlamba inceda ukuphucula ukuchaneka;nangona kunjalo, kwiisampulu zobushushu obuphezulu okanye obuphantsi, ukuhlanjululwa kwengcebiso kunokunciphisa ukuchaneka kwaye abasebenzisi kufuneka bathathele ingqalelo ekhethekileyo.

5. Isantya sokufaka imibhobho: Ukufakwa kwemibhobho kufuneka kuqhutywe ngesantya esigudileyo nesifanelekileyo;ngokukhawuleza kakhulu isantya esinqwenelekayo sinokubangela ukuba isampulu ingene kumkhono, ibangele umonakalo kwipiston kunye nokutywinwa kunye nokungcoliswa komnqamlezo wesampuli.

6. Iingcebiso zokusetyenziswa kwe-pipette
1) Gcina i-posture echanekileyo xa ufaka umbhobho;musa ukubamba i-pipette ngokuqinileyo ngamaxesha onke, sebenzisa i-pipette ngeentambo zomnwe ukunceda ukukhulula ukukhathala kwesandla;tshintsha izandla rhoqo ukuba kunokwenzeka.
2) Khangela isitywina se-pipette rhoqo kwaye ubeke indawo yesitywina ukuba ifunyenwe iyancipha okanye ivuza.
3) Ukulinganisa i-pipette 1-2 ngamaxesha ngonyaka (kuxhomekeke kubuninzi bokusetyenziswa).
4) Kwiipayipi ezininzi, i-piston kufuneka ifakwe i-lubricated ngaphambi nangemva kokusetyenziswa ukugcina isitywina;kwiipipettes zoluhlu oluqhelekileyo, isitywina sikwafanelekile ngaphandle kwe-lubricant.